whitehead institute flow cytometry
Es erlaubt die Analyse von Zellen, die in hoher Geschwindigkeit einzeln an einer elektrischen Spannung oder einem Lichtstrahl vorbeifließen. The flow cytometry lab at the Westmead Institute is part of the Westmead Research Hub core facilities. 2/29/2016 Whitehead Institute for Biomedical Research is a leading, nonprofit research and educational institution that has defined the cutting edge of biomedical science, creating a legacy of research excellence and academic eminence since 1982. provide high quality flow cytometry service and support to the Whitehead Institute investigators and Boston research community. Your generosity can help us bridge this gap and translate laboratory discoveries into clinical practice where they can give new hope. The Babraham Institute Flow Cytometry Core Facility offers high quality service and state-of-the-art instrumentation to members of Babraham Institute and external users. Posting id: 574096346. Wholly independent in its governance, finances and research programs, Whitehead shares a teaching affiliation with Massachusetts Institute of Technology (MIT), offering the intellectual, collegial and scientific benefits of a leading research university. We give our students the opportunity to do important work that is closely linked to the needs of patients. In this process, a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument. This journal also covers relevant techniques such as flow cytometry, image cytometry, beadbased and slide-based array analyses, as well as other cell-based spectroscopic analyses. Sorting is also provided as a service application. Technical Assistant II, Core (Flow Cytometry Core Facility) Whitehead Institute for Biomedical Research. THE DAVID H. KOCH INSTITUTE FOR INTEGRATIVE CANCER RESEARCH AT MIT Whitehead Institute for Biomedical Research New England Cytometry (NEC) BD Spectral Viewer Invitrogen WinMDI Free Flow Software. Eleanor KINCAID, Flow Cytometry Technician of Whitehead Institute for Biomedical Research, Cambridge | Read 15 publications | Contact Eleanor KINCAID Our core facility staff is happy to help you with any questions or concerns you may have. Whitehead Institute, Cambridge, USA We are seeking a Technical Assistant II, Core (Flow Cytometry Core Facility) to join our team! Additional duties include but are not limited to development of proficiency on core facility benchtop analyzers: the BD … Training will be provided, Ability to work flexible hours, including one evening shift per week, Excellent organizational, problem-solving, and communication skills, Ability to work both independently and with a diverse group of people in a customer-service oriented environment. This shared resource is funded in part by NCI Cancer Center Support Grant P30CA16056, NIH Shared Instrument Grant for the ImagestreamX-MKII S10OD018048; … The flow cytometry facility at the Wellcome Sanger Institute is a dedicated scientific service offering investigators with state of art flow cytometry instrumentation together with assistance in running samples, data analysis and experimental design. Flow Cytometry Technician I, Flow Cytometry Core Facility . Cambridge, Massachusetts Position Summary: Whitehead Institute for Biomedical Research is a leading, nonprofit research and educational institution that has defined the cutting edge of biomedical science, creating a legacy of research excellence and academic eminence since 1982. Apply to Merchandise Flow Team Member, Research Associate, Research Specialist and more! The primary duty of the FACS Technician of the BSL2 core cytometry facility is to provide high quality flow cytometry service and support to the Whitehead Institute investigators and Boston research community. OVERALL RESPONSIBILITY. ©1993-2014 Purdue University Cytometry Laboratories, all rights reserved. One of the properties of the cell sorter is the ability not only to detect but to electronically deflect cells which have preset, defined properties into a separate collection tube. PUCL #914 -Flow Cytometry Technician – Whitehead Institute, Cambridge, MA Whitehead Institute for Biomedical Research is a leading, nonprofit research and educational institution that has defined the cutting edge of biomedical science, creating a legacy of … Contact/Staff. Technical Assistant II, Core (Flow Cytometry Core Facility) Whitehead Institute: Cambridge, MA: Research Associate: Precision Life Sciences, LLC: Cambridge, MA: Flow Cytometry Core Facility Manager/Director: Whitehead Institute… He is a pioneer in making transgenic mice, some of which have produced important advances in understanding cancer, neurological and connective tissue diseases, and developmental abnormalities and has explored basic questions such … Koch Institute Flow Cytometry Core at MIT: Home. The sensitivity of these instruments for detecting the presence of molecules expressed at low levels is impressive. Apply to Research Associate, Senior Research Associate, Research Assistant and more! Appointment wait period: If wait periods for any instrument become greater than two weeks, labs with KI or Whitehead Institute affiliation or with NCI funding will be given preference for booking appointments. Students are a fundamental part of research activity at The Westmead Institute. We save lives. Flow cytometry is a very powerful technology which measures various phenotypic, biochemical and molecular characteristics of individual cells (or particles) which have been suspended in a fluid stream. Flow Cytometry (FACS) Technician II Rev. Our research is highly translational. Researchers at Whitehead Institute are world-renowned for their contributions to cancer biology, genomics and more, and the impact of our publications positions us as the top research institution in the world for molecular biology and genetics. It serves over 180 active researchers from over 40 research groups across the Hub and other research locations including Nepean Hospital and the Ingham Institute. WIMR is home to more than 45 research groups concerned with a wide range of human diseases and conditions. As the cells flow past a focused laser beam of appropriate wavelength, the probes’ fluorescence and emitted light is collected and directed to appropriate detectors. Personnel. The technology of flow cytometry is rapidly growing and highly technical. It is widely used in biomedical research, as well as for environmental testing and monitoring. Whitehead Institute, Room 429. All duties will be performed in accordance with the Whitehead Institute’s behavioral and safety standards, mission, values and vision. Whitehead Institute for Biomedical Research is a leading, nonprofit research and educational institution that has defined the cutting edge of biomedical science, creating a legacy of research excellence and academic eminence since 1982. Whitehead Institute for Biomedical Research is a leading, nonprofit research and educational institution that has defined the cutting edge of biomedical science, creating a legacy of research excellence and academic eminence since 1982. The relative size and granularity of a cell is also measured by the flow cytometer because these characteristics can influence the way in which light is scattered as the cell passes through the laser beam. Because up to 21 distinct fluorescent probes reacting with different cell associated molecules can be assessed simultaneously at this facility, cell sorting by flow cytometry can not only separate complex mixtures of cells on the basis of multiple marker expression but collect multiple cell populations (up to 6 populations) at the same time. 509 Flow Cytometry jobs available in Cambridge, MA on Indeed.com. The FACS Technician will possess high level of proficiency on the BD FACSAria SORP and Diva software in order to perform quality high-speed cell sorting. Contact: Patti Wisniewski. Key services. Rudolf Jaenisch is a Founding Member of the Whitehead Institute for Biomedical Research and a Professor of Biology at the Massachusetts Institute of Technology. The Proposed Draft document is undergoing document development committee, expert panel, and active member vote, as well as concurrent Consensus … The FACS Technician will possess high level of proficiency on the BD FACSAria SORP and Diva software in … Flow cytometry typically uses fluorescence probes which bind to specific cell-associated molecules. Cytometry Part B: Clinical Cytometry focuses on the development and applications of cellular system analysis and array based technologies as applied to clinical practice and translational research. 3 Electron Microscopy Core Facility, School of Biological Sciences, Faculty of Biology Medicine and Health, University of Manchester, Manchester M13 9PT, UK. Flow cytometry (FC) is a technique used to detect and measure physical and chemical characteristics of a population of cells or particles.. Our email address will reach all of our staff members, so that any one of us may help you as quickly as possible. Please direct all inquiries about facility instruments or services to our main email address, flowstaff@scripps.edu or our main phone number, (858) 784-8396. It serves over 180 active researchers from over 40 research groups across the Hub and other research locations including Nepean Hospital and the Ingham Institute. FLOW CORE STAFF. The primary duty of the FACS Technician II of the BSL2 core cytometry facility is to provide high quality flow cytometry service and support to the Whitehead Institute investigators and Boston research community. Apply online instantly. WIMR brings together some of the brightest minds to deliver real and significant research breakthroughs for many of the most serious illnesses, improving health and saving lives in Australia and around the world, because people’s lives matter. Related Links . We are passionate about putting our findings into practice and giving hope to those who need it most. MoFlo XDP High Speed Cell Sorter and Analyzer, PUCL Job# 1021 - Flow Cytometry Technician I -Whitehead Institute (Posted 3/8/13), Perform high-speed cell sorting for facility users, Work closely with researchers, maintain high-quality customer relationships, and provide general cell sorting information to researchers. Der Begriff Durchflusszytometrie (Zytometrie = Zell-Vermessung) beschreibt ein Messverfahren, das in der Biologie und in der Medizin zur Anwendung kommt. Whitehead Institute … In addition, facility staff perform cell sorting experiments as well as provide advise and assist researchers with: Keep up-to-date with the latest news from The Westmead Institute, including research findings, new discoveries and stories of hope. For cell purification, flow cytometry is especially well suited for applications requiring high purity (>99%). Core flow cytometry services for investigator and biotech sponsored clinical trials; Publications that include services from FICSR should cite the Cancer Center Support Grant and Shared Instrumentation grants in the acknowledgment section. The flow cytometry lab at the Westmead Institute is part of the Westmead Research Hub core facilities. This facility is centered on flow cytometry, a laser-based technology employed in cell counting, cell sorting, biomarker detection and protein engineering, by suspending cells in a stream of fluid and passing them by … Flowjo Software provide high quality flow cytometry service and support to the Whitehead Institute investigators and Boston research community. The needs of patients are central to everything we do at WIMR. Whitehead's Faculty Members and Fellows run pioneering programs in cancer research, immunology, developmental biology, stem cell research, regenerative medicine, genetics and genomics--programs with a record of success. 2 Flow Cytometry Core Facility, Cancer Research UK Manchester Institute, University of Manchester, Alderley Park, Macclesfield, UK. Welcome to the Swanson Biotechnology Center Flow Cytometry Core Facility The Flow Cytometry Core provides KI and MIT researchers with technical expertise, training and access to sophisticated instrumentation, enabling and supporting the use of a wide range of flow cytometry techniques. Key to our success is the way we connect researchers, clinicians and supporters. While medical research receives support from governments, some worthwhile projects miss out on receiving grants. Technical Assistant I, Core (Flow Cytometry Core Facility) Whitehead Institute, Cambridge, USA. provide high quality flow cytometry service and support to the Whitehead Institute investigators and Boston research community. Whitehead Institute for Biomedical Research is a leading, nonprofit research and educational institution that has defined the cutting edge of biomedical science, creating a legacy of research excellence and academic eminence. Validation of Assays Performed by Flow Cytometry Proposed Draft Please Review and Comment This Proposed Draft document is provided for broad, thorough review in the Clinical and Laboratory Standards Institute (CLSI) consensus review process. You can too. Established in 2000, this flow cytometry facility was completely upgraded in 2012 with an fully automated magnetic cell separator, four international-standard flow cytometry analysers and two high-end cell sorters, offering users cutting- edge cytometry equipment and resources. Director David C. Page likens the Institute to an artists' colony. Instrumentation . Provide high-quality cell sorting to Whitehead Institute investigators, in addition to developing and maintaining a high level of proficiency with all bench-top analyzers, associated software, and peripherals (BD FACSAria IIU SORP, LSRII SORP, BD LSR Fortessa SORP, and BD FACSCalibur with HTS). The Flow and Image Cytometry Resource, initiated in 1988, provides multiparameter flow cytometry services for clinical diagnosis and follow-up to basic and clinical research investigators, carries out an educational outreach program in flow cytometry, and develops new applications and instrumentation. Contact Us . The use of flow cytometry at The Westmead Institute can be divided into two broad categories: analysis and cell sorting. Je nach Form, Struktur und/oder Färbung der Zellen werden unterschiedliche Effekte erzeugt, aus denen die Eigenschaften der Zel… Multiparameter flow cytometry experiment and panel design, Protocols and procedures for new cytometry based assays, Complex cell sorting strategies including single cell sort. Flow Cytometry Technician I, Flow Cytometry Core Facility. Excellent scientific results require exceptional people. Any lab without such affiliation/funding may only book appointments within two weeks Apply for a Whitehead Institute for Biomedical Research Technical Assistant II, Core (Flow Cytometry Core Facility) job in Cambridge, MA. On January 1, 1994, Whitehead Institute Fellow David Bartel officially opened his lab. 4 Manchester Children's Brain Tumour Research Network Royal Manchester Children's Hospital, … 435 Flow Cytometry Core jobs available on Indeed.com. up to 10,000 events per second) makes this technology ideally suited for the reliable and accurate quantitative analysis of selected physical properties of cells of interest. Flow Cytometry Core Facility Analyzer Policy 1. The Westmead Institute’s facility is staffed by two flow cytometry experts committed to providing training and education for all the laboratory users. The FACS Technician will possess high level of proficiency on the BD FACSAria SORP and Diva software in order to perform quality high-speed cell sorting. This includes sample requirements, sample collection requirements, safety requirements, troubleshooting, and reporting issues to manager, Assist with maintenance, quality control, data back-up, and troubleshooting of the sorter and analyzers and report issues to manager, Interact with vendors and field service engineers, as necessary, Assist researchers with scheduling needs, manage and track sorting appointments, Assist with inventory, ordering, and stocking of supplies, Become familiar with flow cytometry journals, associations, and resources, Attend a minimum of one flow cytometry workshop, seminar, vendor show, or core lab visit per year, B.S. Whitehead offers a generous comprehensive benefits package which includes health and dental plans, retirement program, paid time off, reimbursement accounts (FSAs), short and long term disability and much more. degree in biological or physical sciences required, 0 - 1 year of flow cytometry experience. The ability of flow cytometers to evaluate single cells using multiple parameters (up to 20) at an extremely rapid rate (e.g. Methods. Some typical applications include monitoring patients in clinical trials evaluating animals in pre-clinical studies or animal models of malignancy; examining cell-cell interactions; studying the biology of cells and interactions of proteins within cells, finally collecting the cell populations for the further study or diagnostic purposes. Depending on the quality of cell preparations and reagents, as few as 500 molecules per cell may be detected. View this and more full-time & part-time jobs in Cambridge, MA on Snagajob. Whitehead Institute for Biomedical Research is a leading, nonprofit research and educational institution that has defined the cutting edge of biomedical science, creating a legacy of research excellence and academic eminence since 1982.